MBMC Assay of Tabletop Sweeteners Using a Modified Biuret Reagent Lab Report
Experiment 11: Assay of Tabletop Sweeteners Using a
Modified Biuret Reagent
Prelab Questions
- What is the relationship between wave length and energy?
- What is the significance of spectrochemical series?
- Explain crystal field theory.
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According to the spectrochemical series, which of the following Cu atoms will have
bigger separation between the two sets of d orbitals. Explain your reasoning?
Cu atom in Cu2+ poured in to NaOH
Cu atom in Cu2+ poured in to NH3
Which one will absorb light at a higher wavelength? - Why is this method called Modified Biuret Reagent?
- Experiment 12: Agarose gel electrophoresis
Prelab Questions -
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Why it is important to place the gel in the correct direction in the gel cassette while
running it? -
Sketch a gel showing the running pattern of same sized DNA that is,
Linear, circular and circular-supercoiled -
Sketch a gel showing the running pattern of three linear DNA pieces with 100bps, 500bps
and 1000bps - What is the importance of loading dye?
- What is the importance of GelRed?
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Why it is important to place the gel in the correct direction in the gel cassette while
