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PCB 4023 Cell Staining Question

 

  •  Write down the question being addressed by the experiment (1.5 points).

3.)  Create  a  summary  of  the  overall approach you use  to  answer  the  question  being addressed.Do not confuse this section with a material and methods section (1.5 points)

4.) Account for the one by one steps of the method that was used.The easiest way to do that  is by writing “see protocol listed in _______” citing an  online  resource,  specific pages  from  a lab  manual, or  a  published research  paper.  Also,  be  sure  to  note  whether anything you  did deviated  from  that  standard  protocol  by including comments like “We followed the protocol as described except for step 2 where we did _______ instead,” etc. Also, be sure to record any technical errors that occur while doing the procedures, as this saves a lot of confusion later (0.5 points).

5.)  Accurately  record and  show all results yielded  by  the  experiment(raw  data)as the data become available. Based  on  the  raw  results  obtained  (as  a  class),  prepare  an informative graph or table;  you may  choose one  or the other (or both) depending on the experiment and depending  which is the most efficient way to deliver the information.If you get pictures on the microscope, print them out and tape them into the notebook (or if you  cannot,  try  to  briefly  sketch  what  you  saw)(1.0  point). Write  down  a  paragraph  or two describing the results that  you obtained (as a class).Make sure NOT to include any discussion, just plain straight results and also to write down notes about anything unusual you see(1.0 point).

6.)   After   you   have analyzed the   data,   write   1-3   sentences that summarize your interpretation and explanation of the  findings.  Note  that  this  interpretation  section  is the  experiment  and  not  about  what  you  have  learned  or  your  experience  in  the  lab;  it  is not  about  troubleshooting  the  experiment  either,  it  is  about  looking  for  a  scientific explanation for the results obtained (1.5 points).

7.)  Last,  include  a  statement  about possible future scientific directions using as  a  base your findings from the experiment.This is not a section to name the mistakes you did in the experiment; but rather to suggest a future experiment/s using as a base the knowledge obtained  in  the  experiment in  the  lab.  Make  sure  you  are  specific about proposing  the future experiment (1.5 points).

8.) Write here the answers to the questions included at the end of each manual (1 point)

 1.Describe the staining pattern obtained foractin for each of the two cell types tested.Were they the same? Explain.

 2.What types of cell staining did you use today in the lab? Assign each of the 3 stains used with atype of staining methodology.

 3.What type of immunostaining did you use today?Explain your answer.

 4.Name one advantageof using an indirect immunostaininginstead of a directone.

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RC Documentation of Respiratory Assessment Reason for Visit & Health History Essay

 

You will perform a history of a respiratory problem that either your instructor has provided you or one that you have experienced and perform a respiratory assessment. You will document your subjective and objective findings, identify actual or potential risks, and submit this in a Word document to the drop box provided.

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University of Maryland Global Campus Treatment of Cancer Questions

 

Week 2 – This week you are studying acids and bases as you study the chemistry of biology. The measure of the alkalinity (how basic) or acidity (how acidic) of a fluid is accomplished through the use of the pH scale.  Substances that are acidic measure between 0 and 7, while basic substances measure between 7 and 14.  Substances that measure 7 on the pH scale are neither acidic nor basic – they are considered to be neutral.

The billions of the cells that make up the human body are slightly alkaline. The normal pH for human bodies is between 7.35 and 7.45.   The pH levels of our internal body fluids affects every cell in our bodies.  The entire metabolic process depends on a slightly alkaline environment.

An acidic diet, lack of physical exercise, psychological stresses constitute the basis of our modern lifestyle. This unhealthy lifestyle causes acidosis or body over-acidity. Acidosis is a very dangerous condition and it weakens all of our body systems by interrupting all cellular activities and functions. It leads to a general autotoxication of our bodies that gives rise to an internal environment that is conducive to disease.  As such, it is a leading cause of many chronic degenerative diseases. Below is a list of ailments that are rooted in acidosis.

Acid reflux

Cancer

Heart disease

Obesity

Acne

Chronic fatigue

High blood pressure

Osteoporosis

Allergies

Chronic inflammation

Hypercholesterol

Periodontitis

Arteriosclerosis

Diabetes

Immune deficiency

Premature aging

Arthritis

Eczema

Kidney stones

Prostatitis

Bladder conditions

Fungal infections

Mood swings

Underweight

Bronchitis

Headaches

Neuritis

Yeast infections

For this discussion, please do the following:

1. Choose one of the diseases from the list. 

2. Conduct research on the disease you have chosen and using layman’s terms, please discuss in full how acidosis or an acidic body pH leads to the condition you chose.

3. Discuss conventional methods for treating the condition you chose.

4. Offer a reason for why most medical doctors may not consider the pH of patients as both the basis and thus cure for the disease state you have chosen.

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University of Maryland Absence of Enzyme Sucrase and Isomaltase Questions

 

I’m working on a biology discussion question and need an explanation and answer to help me learn.

Week 3 – This week you are studying the biological macromolecules that are instrumental in governing how our bodies function.  One of these macromolecules is a specific protein called an enzyme which also known as a biological catalyst. Enzymes are easily identified by the suffix -ase.

A catalyst is a chemical that speeds up chemical reactions without itself being changed during the course of the reaction. Metabolism is the total of all of the chemical reactions that occur in an organism.  In order formetabolism to occur at speeds necessary for cell functions, a catalyst – or enzyme – is needed.

Each cell in your body contains several thousand different enzymes, most of which are small or large proteins or protein complexes. Most enzymes are highly specific in their action, catalyzing only one type of chemical reaction or, at most, a small number of very similar reactions.  As such, enzymes play a vital role in the proper functioning of our body. Lactase is an example of an enzyme that is needed in order for us to properly digest the sugar lactose, which is found in dairy products. Individuals who lack this enzyme are unable to digest or breakdown this sugar and therefore cannot eat dairy without experiencing side effects.

Research and share with the class a human condition that is the result of the malfunctioning of an enzyme, the absence of an enzyme, or low levels of an enzyme. 

For your chosen condition, please include the following:

1) The name of the condition

2) Name of the specific enzyme involved in the condition 

3) Whether the enzyme is lacking, whether it malfunctions,  or whether its levels are low

4) The symptoms of the disease or condition

5) Whether or not there is a treatment or cure for the disease or condition

6) The life expectancy for someone who has the disease you have chosen

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University of Maryland Week 5 Biotechnology Industry Organization Questions

 

Week 5 – Biotechnology harnesses cellular and biomolecular processes to develop technologies and products that help improve our lives and the health of our planet. We have used the biological processes of microorganisms for more than 6,000 years to make useful food products, such as bread and cheese, and to preserve dairy products.

According to the Biotechnology Industry Organization, “Modern biotechnology provides breakthrough products and technologies to combat debilitating and rare diseases, reduce our environmental footprint, feed the hungry, use less and cleaner energy, and have safer, cleaner and more efficient industrial manufacturing processes.” As such, biotechnology can be used to:

a. Heal the World – healthcare

b. Fuel the World – industrial and environmental

c. Feed the World – food and agriculture

For this discussion, please respond to the following questions:

1. Which area of biotechnology (feed the world, fuel the world, heal the world) do you believe is the most important for society today? Explain your choice.

2. Identify and research a specific application of biotechnology from within the category you picked in number 1.

a. Describe in detail its use

b. Describe its impact on society.

3. Cite all references

Week 6 – The living organisms that exist upon the earth exhibit a wide array of physical, chemical, and behavioral variation. Each feature of an organism is an adaptation acquired by that species through evolution, which uniquely suits an organism to survival within a given set of environmental conditions.

Find an example of the weirdest, wildest, most bizarre or interesting species you can. Discuss those features that uniquely suit this organism to survival within a given set of environmental conditions. When considering the features of an organism, include its observable physical characteristics (which can be seen by the naked eye), as well as the molecular, metabolic, or chemical characteristics of the organism that can occur at the level of (or within) cells. You can draw examples from the textbook or search the Web for the organism of your choice. If you can find it on the web, include a link to a picture or description of your organism. Also include links/references if appropriate.

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Ohio State University The freezing point depression of a solution Question

 

I’m working on a chemistry question and need an explanation to help me learn.

What is the freezing point of a 0.2 mL solution that consists of 1 mg of glucagon, 11.1 mg of trehalose dihydrate, and 1.2 mg of sulfuric acid in a dimethyl sulfoxide diluent?

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TCC Nuclear Fission Nuclear Chain Reaction & Balancing Reaction Exercises

 

Question 7 :Fluorine-20 has a half-life of 11.0 ss. If a sample initially contains 28.0 μgμg of this isotope, how much remains after 44.0 ss ?

Express your answer in micrograms to three significant figures.

question 4 : Radium-223 nuclei usually decay by alpha emission. Once in every billion decays, a radium-223 nucleus emits a carbon-14 nucleus.

Part A : Write a balanced nuclear equation for alpha emission.

Part B: Write a balanced nuclear equation for carbon-14 emission.

ANSWER THE FOLLOWING QUESTION ATTACHED AS WELL